For research purposes only. Not for human consumption.
How to Reconstitute Peptides (Clean, Accurate, Repeatable)
This guide outlines standard laboratory handling steps for reconstituting lyophilized peptide powders and calculating final solution concentration for research use.
Shop: Hospira Bacteriostatic Water
What Reconstitution Means
Many peptides are supplied as lyophilized powders. Reconstitution refers to adding a measured volume of diluent to create a solution with a known concentration. Accuracy depends on clean technique and correct math.
What You Need
- Lyophilized peptide vial (labeled mass in mg)
- Bacteriostatic water
- Sterile syringe or pipette for measurement
- Alcohol wipes
Step-by-Step Reconstitution
- Clean vial stoppers with alcohol and allow to dry.
- Measure the desired volume of bacteriostatic water.
- Slowly introduce liquid down the vial wall.
- Gently swirl until fully dissolved. Do not shake.
- Label vial with date and calculated concentration.
Common errors
- Confusing mg and mcg
- Incorrect diluent volume
- Shaking the vial
- Failing to document concentration
This information is provided for laboratory and research handling only.